Fig 1: (A) ANXA8 is up-regulated in GC tissues in comparison with ANGTs in TCGA (tumor, n=408, normal, n=211, *P<0.05). (B-C) ANXA8 was highly expressed in GC tissues compared with normal gastric tissues in GEO (GSE13861, tumor, n=65, normal, n=19, *P=0.032; GSE19826, tumor, n=10, normal, n=14, *P=0.023). (D) The relative expression of qRT-PCR results between tumor (n=58) and normal (n=58), ****P<0.0001. All *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Overall survival (E) and disease-free survival (F) curves for the GC patient groups with low (n=192) and high (n=188) ANXA8 expression. (G) Western blot results showed that the levels of ANXA8 were higher in six pairs of GC patients tumor tissues compared with ANGTs.
Fig 2: Overall survival (A) and disease-free survival (B) curves for the GC patient groups with low (n=63) and high (n=89) ANXA8 expression. A worse prognosis of high ANXA8 (n=19) expression compared with low ANXA8 (n=28) was observed in OS (p=0.01) and DFS (p=0.02) for early TNM stage (C and D). Significant differences were found between high ANXA8 expression (n=70) and low ANXA8 (n=35) expression in OS (p<0.001) and DFS (p=0.001) for late TNM stage (E and F).
Fig 3: ANXA8 expression in GC tissues and ANGTs. IHC staining showed low ANXA8 expression in normal gastric tissues (A and B) and high (C and D), medium (E and F) and low (G and H) GC tissues.
Fig 4: Differentially expressed Annexins were associated with prognosis of RCC patientsPrognostic values of differentially expressed Annexins in RCC. Prognostic significance of (A)ANXA1, (B)ANXA2R, (C)ANXA3, (D)ANXA4, (E)ANXA8, (F)ANXA8L1, (G)ANXA9 and (H)ANXA13 in RCC. The p-value was calculated using the Log-rank testRCC, renal cell carcinoma; HR, hazard ratio
Fig 5: Knockdown of ANXA8 mainly influenced the cell cycle in 769-P(A) Down and (B) up regulated KEGG pathways of the differentially expressed genes in 769-P transfected with shANXA8 compared with shCtrl. (C) Representative cell cycle distribution by propidium staining in 769-P transfected with shCtrl. (D) Representative cell cycle distribution by propidium staining in 769-P transfected with shANXA8. (E) Quantitative analysis of proportion of the cells in different phases between 769-P transfected with shANXA8 and shCtrl. ****p < 0.0001, ***p < 0.001
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